Re: Counting Cells

Posted by Robert Smith on
URL: http://imagej.273.s1.nabble.com/Counting-Cells-tp5020676p5020685.html

Just a suggestion but if you go ahead and threshold the image you can use analyse  particles. that will usually count all the particles in the image.

I hope I understand what it is you are asking, but this always works for me.

Bob


________________________________
From: Curtis Rueden <[hidden email]>
Sent: Thursday, May 17, 2018 11:49 AM
To: [hidden email]
Subject: Re: Counting Cells

Hi Nadine,

Please take a look at https://imagej.net/Segmentation for an overview of
Segmentation - ImageJ<https://imagej.net/Segmentation>
imagej.net
Image segmentation is "the process of partitioning a digital image into multiple segments." (Wikipedia)



automated segmentation and counting techniques in ImageJ.

Regards,
Curtis


On Wed, May 16, 2018, 08:16 Nadine Nahra <[hidden email]> wrote:

> Good Morning,
>
> I am working on a project with mice breast tissue, the slides have been
> stained using Immunohistochemistry IHC staining.
> Is there any tool on ImageJ that I can use to count nuclei thats not by
> hand? Not sure who to contact about this but if anyone has answers it is
> greatly appreciated.
> Thanks!
>
> -Nadine
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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