How to align images manually and merge them into a multi-channel composite image?

Posted by Jiseok on May 22, 2018; 8:19pm
URL: http://imagej.273.s1.nabble.com/How-to-align-images-manually-and-merge-them-into-a-multi-channel-composite-image-tp5020699.html

Hi,

I have 4 images taken in 4 different days.

I want to manually align the 4 images, because there are small shifts and
movements each time I image.

Then I want to merge the 4 aligned images into a 4-channel composite image,
where each day image corresponds to a different channel. For example,
day1=R, day2=G, day3=B, day4=gray.

Can anyone kindly give me any advice how I can do this?

I don’t think I can use any automatic method for aligning, since the images
are quite complicated and need human eye inspection to decide matching
points.

I attached the 4 images, if anyone wants to see what they’re like.

Thank you very much!

Day1
<http://imagej.1557.x6.nabble.com/file/t381950/day1.jpg>

Day2
<http://imagej.1557.x6.nabble.com/file/t381950/day2.jpg>

Day3
<http://imagej.1557.x6.nabble.com/file/t381950/day3.jpg>

Day4
<http://imagej.1557.x6.nabble.com/file/t381950/day4.jpg>



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