Re: How to align images manually and merge them into a multi-channel composite image?

Posted by Kenneth Sloan-2 on May 22, 2018; 11:16pm
URL: http://imagej.273.s1.nabble.com/How-to-align-images-manually-and-merge-them-into-a-multi-channel-composite-image-tp5020699p5020702.html

I recommend Plugins->Registration->Align Image by Roi.

--
Kenneth Sloan
[hidden email]
Vision is the art of seeing what is invisible to others.

> On 22 May 2018, at 15:19 , Jiseok <[hidden email]> wrote:
>
> Hi,
>
> I have 4 images taken in 4 different days.
>
> I want to manually align the 4 images, because there are small shifts and
> movements each time I image.
>
> Then I want to merge the 4 aligned images into a 4-channel composite image,
> where each day image corresponds to a different channel. For example,
> day1=R, day2=G, day3=B, day4=gray.
>
> Can anyone kindly give me any advice how I can do this?
>
> I don’t think I can use any automatic method for aligning, since the images
> are quite complicated and need human eye inspection to decide matching
> points.
>
> I attached the 4 images, if anyone wants to see what they’re like.
>
> Thank you very much!
>
> Day1
> <http://imagej.1557.x6.nabble.com/file/t381950/day1.jpg>
>
> Day2
> <http://imagej.1557.x6.nabble.com/file/t381950/day2.jpg>
>
> Day3
> <http://imagej.1557.x6.nabble.com/file/t381950/day3.jpg>
>
> Day4
> <http://imagej.1557.x6.nabble.com/file/t381950/day4.jpg>
>
>
>
> --
> Sent from: http://imagej.1557.x6.nabble.com/
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html