Posted by
Stephan.Preibisch@mdc-berlin.de on
May 23, 2018; 6:38am
URL: http://imagej.273.s1.nabble.com/How-to-align-images-manually-and-merge-them-into-a-multi-channel-composite-image-tp5020699p5020709.html
Hi,
This can be automatically aligned using the Descriptor-based series registration … check the result here:
http://preibischlab.mdc-berlin.de/download/aligned.zipUsing these parameters (for longer time series use regularization with a rigid model)
http://preibischlab.mdc-berlin.de/download/params.zipAll the best,
Stephan
---
Dr. Stephan Preibisch
Group Leader
Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine (MDC)
Building 89, 1.08b
Robert-Rössle-Str. 10
13125 Berlin
email:
[hidden email]<mailto:
[hidden email]>
web:
http://preibischlab.mdc-berlin.detwitter:
http://twitter.com/preibischsOn May 22, 2018, at 10:19 PM, Jiseok <
[hidden email]<mailto:
[hidden email]>> wrote:
Hi,
I have 4 images taken in 4 different days.
I want to manually align the 4 images, because there are small shifts and
movements each time I image.
Then I want to merge the 4 aligned images into a 4-channel composite image,
where each day image corresponds to a different channel. For example,
day1=R, day2=G, day3=B, day4=gray.
Can anyone kindly give me any advice how I can do this?
I don’t think I can use any automatic method for aligning, since the images
are quite complicated and need human eye inspection to decide matching
points.
I attached the 4 images, if anyone wants to see what they’re like.
Thank you very much!
Day1
<
http://imagej.1557.x6.nabble.com/file/t381950/day1.jpg>
Day2
<
http://imagej.1557.x6.nabble.com/file/t381950/day2.jpg>
Day3
<
http://imagej.1557.x6.nabble.com/file/t381950/day3.jpg>
Day4
<
http://imagej.1557.x6.nabble.com/file/t381950/day4.jpg>
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