Posted by
Krs5 on
May 24, 2018; 7:24am
URL: http://imagej.273.s1.nabble.com/Color-thresholding-and-obtaining-Lab-values-tp5020700p5020724.html
Dear Rie,
To measure only your selection you can use:
- Edit > Selection > Add to Manager
- in the ROI Manager menus select More > Multi Measure
- in the menu presented select "Measure all 3 slices" and deselect "One row per slice"
I hope this solves your problem.
Best wishes
Kees
Dr Ir K.R. Straatman
Senior Experimental Officer
Advanced Imaging Facility
Centre for Core Biotechnology Services
University of Leicester
www.le.ac.uk/advanced-imaging-facility
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Rie Sadohara
Sent: 22 May 2018 22:13
To:
[hidden email]
Subject: Color thresholding and obtaining Lab values
Hello all,
I am trying to get Lab values from images of yellow beans. There are some black and white parts in the image, so I want to remove those parts and measure the yellowness of the bean seeds. Below are the steps I took, but Lab values are calculated from the entire image. Could someone tell me how to do it right?
1. Open --> Open my photo, which is an RGB color image.
2. Image --> Adjust --> Color threshold... --> Then set the threshold of L* value to be 71-216 so that the black and white parts will be excluded. --> Select.
3. The yellow parts (of which I want to know the Lab values) are circled with yellow lines now.
4. Image --> Type --> Lab stack.
5. Analyze --> Measure. for each stack (L, a, and b) 6. The returned Lab values are based on the entire image. It works as though there had been no color thresholding.
My understanding is that the parts that are circled with bright yellow lines after color thresholding are the area that are going to be used for Lab measurements? (Please see the attachment. In the image, the yellow parts (my ROI) are segregated correctly, but how can I get Lab values out of this region?)
Any advice would be greatly appreciated,
Rie
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