Posted by
Cammer, Michael on
May 31, 2018; 5:09pm
URL: http://imagej.273.s1.nabble.com/distance-measurement-and-cropping-behavior-questions-tp5020756.html
I was wondering whether anybody else is having odd behaviors with ImageJ 1.52d8 with Java 1.8 on Windows 7? One of them may be my fault and want to confirm; the other is something odd with the software.
1.
When I do a line profile plot and measure a distance on it, most of the time the distance returned is incorrect.
I thought that when the images were spatially calibrated, the plot also was supposed to return the calibrated length. Am I mistaken about this or is this a bug? What makes me puzzled is that the lengths reported are inconsistent.
2.
When I draw a rectangle on an image and then crop, the upper left of the image is cropped instead of the ROI. The resultant image is the size of the ROI, but not the correct location.
When I duplicate, the correct region is duplicated.
These are 4096 x 4096 pixels 16 bit and 32bit images generated with a Talos TEM and Gatan camera. When the images are converted to RGB or 8 bit, this problem does not happen.
Any help appreciated.
Thank you.
Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016
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