Re: Problem with creating ring like ROIs with particle analyzer

Posted by Gabriel Landini on Jul 23, 2018; 10:22am
URL: http://imagej.273.s1.nabble.com/Problem-with-creating-ring-like-ROIs-with-particle-analyzer-tp5020989p5020991.html

On Monday, 23 July 2018 08:00:14 BST Straub, Volko A. (Dr.) wrote:
> However, when
> these regions are added to the ROI manager, the ROIs now include the
> central hole rather than just the ring. So, any subsequent measurements
> using these ROIs measure not just the ring but the ring plus the hole.

This will not strictly answer your question on ROIs but might be a useful
alternative if you do not find a solution.
Rather than using ROIs I prefer using a binary mask of the objects to analyse
and get the pixel data via Redirection. I use Particles 8 for this, it
restricts the data collection to the pixels set in white in the mask. It you
chose redirection it automatically gets the pixel stats (gresycale or RGB).

Cheers
Gabriel

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