CZI to tiff Scaling problem

Posted by Kira Rienecker on
URL: http://imagej.273.s1.nabble.com/CZI-to-tiff-Scaling-problem-tp5021024.html

Hello everyone!


I've been using DSRichardson's czi to tiff conversion macro for Image J Bioformats and one particular file is giving me problems with scaling. All of the settings for pixel size seem to be correct (0.441 µm^2 per pixel), but the measurements are coming out ~1/4 the size they should be.


I haven't had this problem with ANY of the other files from the same scan day (Zeiss Axioscan) and I have scanned this slide alone on a separate day and I still get the same result.


Any ideas?

I appreciate the help!


-Kira Rienecker

Final year PhD student

(MacBook Pro user)


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