Screen thresholded objects for in/out of focus

Posted by TimFeinstein on Aug 06, 2018; 7:54pm
URL: http://imagej.273.s1.nabble.com/Screen-thresholded-objects-for-in-out-of-focus-tp5021054.html

Hello,

I have a field of non-touching fluorescent objects that are segmented by thresholding, and I want to discard objects which are out of focus.  In-focus objects (cilia) should have a clear and straight edge that an algorithm like Modulation Transfer Function should be able to detect.  However, MTF plugins are overkill for my needs and they don't seem like they'd be easy to incorporate into a macro script.  I need a single number that represents the sharp-ness or focused-ness of each cilium, so that a macro can automatically discard fuzzy cilia before measuring them.  I like, for example, the Gaussian gradient function in Matlab, but I'm not sure something similar is available in ImageJ.  

Any suggestions would be appreciated.  Thanks!


TF

Timothy Feinstein, Ph.D.
Research Scientist
Department of Developmental Biology
University of Pittsburgh

 



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