Substack and composite

Posted by Houssin, Nathalie Samantha Laetitia on
URL: http://imagej.273.s1.nabble.com/Substack-and-composite-tp5021285.html

Hello,

It might seem a very basic thing to do but I would like some advice on generating a composite from a stack :
I tried to make it like this:

  1.  Open .oir file in FIJI (using Pluging > OlympusViewer>Viewer)
  2.  Select z-section
     *   Image>Stacks>Tools>Make Substack
     *   Enter section number (z-value)
When I do this, an exception message appears :

java.lang.IllegalArgumentException: Channel out of range
                at ij.CompositeImage.setChannelLut(CompositeImage.java:538)
                at ij.plugin.SubHyperstackMaker.makeSubhyperstack(SubHyperstackMaker.java:121)
                at ij.plugin.SubHyperstackMaker.makeSubhyperstack(SubHyperstackMaker.java:60)
                at ij.plugin.SubHyperstackMaker.run(SubHyperstackMaker.java:50)
                at ij.plugin.SubstackMaker.run(SubstackMaker.java:33)
                at ij.IJ.runPlugIn(IJ.java:198)
                at ij.Executer.runCommand(Executer.java:137)
                at ij.Executer.run(Executer.java:66)
                at java.lang.Thread.run(Thread.java:745)

And I can't generate a composite file. Any tip on how to proceed will be welcome

Thank you for your time,

Bests,

Nathalie

Nathalie HOUSSIN

Plageman's Lab
Fry Hall room 350
338 W 10th avenue
Columbus, Ohio 43210
Tel: [phone]


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