Posted by
Straub, Volko A. (Dr.) on
Oct 21, 2018; 6:15am
URL: http://imagej.273.s1.nabble.com/Analyze-particles-and-show-circles-tp5021328p5021330.html
Sorry, forgot to attached the macro code to my previous post. Here it is:
run("Blobs (25K)");
setAutoThreshold("Default");
setOption("BlackBackground", true);
run("Convert to Mask");
run("Watershed");
run("Convert to Mask");
run("Ultimate Points");
setThreshold(1, 255);
run("Convert to Mask");
run("Mean...", "radius=3");
setThreshold(1, 255);
run("Convert to Mask");
________________________________
From: JCortex <
[hidden email]>
Sent: 19 October 2018 17:23:30
To:
[hidden email]
Subject: Analyze particles and show circles?
Hello,
I have thresholded images of Nissl-stained cortical sections that I need to
process.
Essentially, I need to sort the cells/objects by size and then create an
overlay/second image of the sorted objects, but instead of displaying the
objects, I need it to display filled circles (of a uniform size) at the
location of each identified object.
The closest I've come to achieving this so far with the program is to use
'analyze particles', select my size range, and then show either 'ellipses',
'masks', or 'overlay masks'. 'Ellipses' is the closest to what I need except
they are ellipses, not filled circles, and they all have varying sizes,
whereas I need to be able to set a uniform size for all sorted objects.
Is there any way to achieve this?
Thank you
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