Posted by
Houssin, Nathalie Samantha Laetitia on
Oct 30, 2018; 8:37pm
URL: http://imagej.273.s1.nabble.com/Issue-with-the-NDi6d-plugin-Bio-Formats-Importer-tp5021378.html
Hello everyone,
I am currently working with the ImageJ 1.52h version and I can't upload my .nd2 as a metadata document or I generate stacks with a mix or dark and grayscale images
In brief, when I choose the NDi6d plugin --> ND to image6D, this window pops:
(Fiji Is Just) ImageJ 2.0.0-rc-68/1.52h; Java 1.8.0_66 [64-bit]; Windows 7 6.1; 114MB of 12119MB (<1%)
java.lang.UnsatisfiedLinkError: no Nd2SdkWrapperI6D in java.library.path
at java.lang.ClassLoader.loadLibrary(ClassLoader.java:1864)
at java.lang.Runtime.loadLibrary0(Runtime.java:870)
at java.lang.System.loadLibrary(System.java:1122)
at NDi6d.ND_to_Image6D.<clinit>(ND_to_Image6D.java:44)
at sun.reflect.NativeConstructorAccessorImpl.newInstance0(Native Method)
at sun.reflect.NativeConstructorAccessorImpl.newInstance(NativeConstructorAccessorImpl.java:62)
at sun.reflect.DelegatingConstructorAccessorImpl.newInstance(DelegatingConstructorAccessorImpl.java:45)
at java.lang.reflect.Constructor.newInstance(Constructor.java:422)
at java.lang.Class.newInstance(Class.java:442)
at ij.IJ.runUserPlugIn(IJ.java:226)
at ij.IJ.runPlugIn(IJ.java:192)
at ij.Executer.runCommand(Executer.java:137)
at ij.Executer.run(Executer.java:66)
at java.lang.Thread.run(Thread.java:745)
I also tried to import an image sequence unsuccessfully (File> Import> Image Sequence) as I exported my nd2 files as .tif
Lastly, I tried to use the Bio-Formats Importer but it seems that it is not able to recognize the amount of channels and it is generating stacks of dark images
I've uploaded one of my files on dropbox if someone has any idea on why it is not working
https://www.dropbox.com/s/4apvugdc649n8k9/x40o_E10%2C5_Par202--_myoIIb488_Afadin594_1.nd2?dl=0Thank you for any feedback you can provide,
Bests,
Nathalie
Nathalie HOUSSIN
Plageman's Lab
Fry Hall room 350
338 W 10th avenue
Columbus, Ohio 43210
Tel: 614-292-0636
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