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Re: Issue with the NDi6d plugin/ Bio-Formats Importer

Posted by Herbie on Oct 30, 2018; 9:28pm
URL: http://imagej.273.s1.nabble.com/Issue-with-the-NDi6d-plugin-Bio-Formats-Importer-tp5021378p5021380.html

Nathalie,

I didn't check anything in the BioFormats-dialog (BioFormats-Importer).
View stack with: Hyperstack
Color mode: Default
Nothing else checked and it opens just fine with
3 color channels and 15 z-slices.

I use plain ImageJ 1.52h with Java 1.8.0_172 and the BioFormats-plugin
installed.

Please try again and be careful with the settings.

Regards

Herbie

::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
Am 30.10.18 um 22:17 schrieb Houssin, Nathalie Samantha Laetitia:

> Herbie,
> Thank you for checking it.
>
> I tried it again using BIoFormat and have a message :
> Warning: Data has too many channels for Default color mode
>
> I imagine that I have to change the parameters set in the dialog box of the Bioformat
> Thank you also for the tip for the color display. I have indeed stacks of black slices not corresponding to any channel. I'd probably have to reinstall the plugin
>
> Again, thank you,
>
> Nathalie
>
> -----Original Message-----
> From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Herbie
> Sent: Tuesday, October 30, 2018 5:02 PM
> To: [hidden email]
> Subject: Re: Issue with the NDi6d plugin/ Bio-Formats Importer
>
> Good day Nathalie,
>
> I've just opened your sample stack using BioFormats and, as far as I can judge, it opens nicely.
>
> If you see seemingly black slices, then please go to
>     "Image >> Adjust >> Brightness/Contrast..."
> and click "Reset".
>
> Please read here
> <https://forum.image.sc/t/imagej-overexposes-images/20403/2?u=herbie>
> what is the cause and what the "Reset" actually does.
>
> Regards
>
> Herbie
>
> :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
> Am 30.10.18 um 21:37 schrieb Houssin, Nathalie Samantha Laetitia:
>> Hello everyone,
>>
>> I am currently working with the ImageJ 1.52h version and I can't
>> upload my .nd2 as a metadata document or I generate stacks with a mix or dark and grayscale images In brief, when I choose the NDi6d plugin --> ND to image6D, this window pops:
>>
>> (Fiji Is Just) ImageJ 2.0.0-rc-68/1.52h; Java 1.8.0_66 [64-bit];
>> Windows 7 6.1; 114MB of 12119MB (<1%)
>> java.lang.UnsatisfiedLinkError: no Nd2SdkWrapperI6D in java.library.path
>>                   at java.lang.ClassLoader.loadLibrary(ClassLoader.java:1864)
>>                   at java.lang.Runtime.loadLibrary0(Runtime.java:870)
>>                   at java.lang.System.loadLibrary(System.java:1122)
>>                   at NDi6d.ND_to_Image6D.<clinit>(ND_to_Image6D.java:44)
>>                   at sun.reflect.NativeConstructorAccessorImpl.newInstance0(Native Method)
>>                   at sun.reflect.NativeConstructorAccessorImpl.newInstance(NativeConstructorAccessorImpl.java:62)
>>                   at sun.reflect.DelegatingConstructorAccessorImpl.newInstance(DelegatingConstructorAccessorImpl.java:45)
>>                   at java.lang.reflect.Constructor.newInstance(Constructor.java:422)
>>                   at java.lang.Class.newInstance(Class.java:442)
>>                   at ij.IJ.runUserPlugIn(IJ.java:226)
>>                   at ij.IJ.runPlugIn(IJ.java:192)
>>                   at ij.Executer.runCommand(Executer.java:137)
>>                   at ij.Executer.run(Executer.java:66)
>>                   at java.lang.Thread.run(Thread.java:745)
>>
>> I also tried to import an image sequence unsuccessfully (File> Import>
>> Image Sequence) as I exported my nd2 files as .tif Lastly, I tried to
>> use the Bio-Formats Importer but it seems that it is not able to
>> recognize the amount of channels and it is generating stacks of dark
>> images
>>
>> I've uploaded one of my files on dropbox if someone has any idea on
>> why it is not working
>>
>> https://www.dropbox.com/s/4apvugdc649n8k9/x40o_E10%2C5_Par202--_myoIIb
>> 488_Afadin594_1.nd2?dl=0
>>
>> Thank you for any feedback you can provide,
>>
>> Bests,
>>
>> Nathalie
>>
>> Nathalie HOUSSIN
>>
>> Plageman's Lab
>> Fry Hall room 350
>> 338 W 10th avenue
>> Columbus, Ohio 43210
>> Tel: 614-292-0636
>>
>>
>> --
>> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>>
>
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>

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