To normalize Pixel intensities allowing for differing pixel/micron ratios

Posted by Trevor Grigg on
URL: http://imagej.273.s1.nabble.com/To-normalize-Pixel-intensities-allowing-for-differing-pixel-micron-ratios-tp5021435.html

How can you normalize the mean gray value of pixel intensities 8  bit (0-255)taken of cells when the images are at different magnifications 400x or 630x  with pixels/micron being at 3.723 for some images and 5.882 for other images. I am measuring the mean pixel intensity of each cell (ROI) and the distribution of these means I am using to form a box and whisker chart to measure the fluorescence of my peptide within the population of cells. I am new to using Image J but I’m on a fast learning curve and I’m asking if someone can please assist me. Thank you

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