Re: Separating intracellular objects from extracellular objects

Posted by María Victoria Pepe on Nov 26, 2018; 11:58am
URL: http://imagej.273.s1.nabble.com/Separating-intracellular-objects-from-extracellular-objects-tp5021479p5021501.html

Hi Kees!!

I´ve just downloaded the plugin, so I´ll try it soon

Thanks for your answer.

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El jue., 22 nov. 2018 a las 5:40, Straatman, Kees (Dr.) (<
[hidden email]>) escribió:

> Dear Maria,
>
> Would the 3D watershed or the 3D Spot Segmentation be of use? For both
> see:
> http://imagejdocu.tudor.lu/doku.php?id=plugin:segmentation:3d_spots_segmentation:start
> and they are part of the 3D ImageJ suite.
>
> Best wishes
>
> Kees
>
>
> Dr Ir K.R. Straatman
> Senior Experimental Officer
> Advanced Imaging Facility
> Centre for Core Biotechnology Services
> University of Leicester
> www.le.ac.uk/advanced-imaging-facility
>
> -----Original Message-----
> From: María Victoria Pepe <[hidden email]>
> Sent: 20 November 2018 15:12
> To: [hidden email]
> Subject: Separating intracellular objects from extracellular objects
>
> Hi
>
> I´m studying efferocytosis of apoptotic cells by epithelial cells, for
> which I´m using the *3D Objects Counter* tool. I make a map of the
> apoptotic cell channel and then, I merge this map with the reslice of the
> epithelial cells channel. Then I decide if the object in the map is inside
> or outside the cell.  This is a slow process but generally it works. But
> there are some problems when I have two objects close to each other, one
> outside and one inside, because sometimes they are consider like one only
> object.
> How can I generate a solid mask using the reslice of the epithelial cells
> channel to use it to separate intracellular objects from extracellular
> objects?
>
>
> I attached one image as an example.
>
>
>
> Thanks for your time
>
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