Particle segmentation

Posted by James Wesley-Smith on Jan 22, 2019; 7:30am
URL: http://imagej.273.s1.nabble.com/Particle-segmentation-tp5021680.html

Me again.

To familiarise myself further with FIJI,  I deliberately chose gold
nanorods for their electron opacity and thus contrast, but I am struggling
to segment them. Thresholding successfully  separates them from the
background, but I have tried various settings of erode / dilate and open /
close routines to no avail - just cannot separate them completely. I even
looked for watershed segmentation, which tends to "over segment". I also
looked at answers to previous questions posted on this listserver without
much success.

 

Where am I going wrong? What segmentation steps would you suggest I follow
for this sort of sample (attached)?

 

 

Thanks again!

 

 

Best regards,

James

 


--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html