Re: Particle segmentation

Posted by Michael Schmid on Jan 22, 2019; 5:04pm
URL: http://imagej.273.s1.nabble.com/Particle-segmentation-tp5021680p5021686.html

Dear James,

In your image, there is an outlier pixel at position (667, 281), with a
pixel value of 171 (the surrounding pixels are in the range 70-80).

This pixel acts as a seed for a dividing line of the Adjustable Watershed.

You can use, e.g. Process>Noise>Remove Outliers. For single outlier
pixels, a radius of 0.5 is enough; and specify a sufficiently large
threshold to avoid affecting the gaps between the particles, e.g. 50.

[BTW, please make sure you reply to the correct thread; otherwise it is
impossible to find things in the list archives]

Best,

Michael
________________________________________________________________

On 22.01.19 12:52, Jaime Wesley-Smith wrote:
 > Dear Michael
 > I think the Adjustable Watershed does a pretty nice job (with the
exception
 > of particles 23-25) on the image attached! Hope I have recorded the macro
 > correctly for you to see...
 >
 > I will also try your suggestion of the overlay.
 >
 > Many thanks again!
 >
 > Best regards,
 >
 > James

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