Re: Questions about channelunmixing using Poisson NMF

Posted by Jan Kovac on Jan 29, 2019; 4:44pm
URL: http://imagej.273.s1.nabble.com/Questions-about-channelunmixing-using-Poisson-NMF-tp5020272p5021732.html

Dear Stoyan
This is quite older post but I have a similar problem with the same plugin
"Poisson NMF". Did you recently solved the described problem? Or you have
some solution? In my opinion, the problem is hidden in java code and is not
easy for me to modify it. If I choose as a source stack some e.g. Leica
lambda stack it is working, but if I am using Olympus .oif files without
saved wavelenght in metadata, it is immposible to unmix spectra in real
wavelenght. Also NaN "values" appears. I am just asking if you find some
solution.

Jan



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