Re: Questions about channelunmixing using Poisson NMF

Posted by Stoyan Pavlov on Jan 31, 2019; 1:25am
URL: http://imagej.273.s1.nabble.com/Questions-about-channelunmixing-using-Poisson-NMF-tp5020272p5021736.html

Hi Jan,
Unfortunately I didn't receive any answer in this otherwise very active and
helpful community.
I just couldn't continue wasting time with this at the moment,and let it
go. However the problem still stands and if you find a solution or someone
on the ImageJ list is kind enough to offer help on this issue I would much
appreciate it.
Stoyan


На вт, 29 ян. 2019 г., 18:55 Jan Kovac <[hidden email] написа:

> Dear Stoyan
> This is quite older post but I have a similar problem with the same plugin
> "Poisson NMF". Did you recently solved the described problem? Or you have
> some solution? In my opinion, the problem is hidden in java code and is not
> easy for me to modify it. If I choose as a source stack some e.g. Leica
> lambda stack it is working, but if I am using Olympus .oif files without
> saved wavelenght in metadata, it is immposible to unmix spectra in real
> wavelenght. Also NaN "values" appears. I am just asking if you find some
> solution.
>
> Jan
>
>
>
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