Macro Error Message from makeLine function - no more than 200 points

Posted by Aliciaddn on Jan 31, 2019; 3:36pm
URL: http://imagej.273.s1.nabble.com/Macro-Error-Message-from-makeLine-function-no-more-than-200-points-tp5021737.html

Hi all,

I am segmenting cells from microscopy images with a custom macro using
squassh.
I then record all the pixel coordinates of the cell contour and create an
ROI of the snake thanks to makeLine.
There is a linewidth of few pixels that I define also to by able to measure
fluorescence a bit inside my cell.

<http://imagej.1557.x6.nabble.com/file/t382224/Snake.png>

My macro is working fine when the contour of my cell is smaller than 200
points but above that threshold, I receive an error message saying:

<http://imagej.1557.x6.nabble.com/file/t382224/Macro_Error.bmp>

From what I searched, it's seems this limit of 200 points is applied in
makePolygon. But do you know if it's possible to change this limit of 200
points in makeLine ?

ps: I thought of splitting my line in two but I would like to avoid that
because I am then measuring local curvatures and it would make the work and
post-processing much more complicated.
I thought also to downsized the image but I really would like to avoid that
too not to loose resolution.

Thank you for your answers,

Best,
Alicia



--
Sent from: http://imagej.1557.x6.nabble.com/

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html