Re: Quantifying overlap on fluorescence image
Posted by
Nicholas George on
Apr 05, 2019; 4:25pm
URL: http://imagej.273.s1.nabble.com/Quantifying-overlap-on-fluorescence-image-tp5021990p5021992.html
Hello Rohitesh, welcome to the ImageJ community.
For pixel based colocalization, you can use this imageJ/Fiji plugin
https://imagej.net/Coloc_2 . The website explains some of the caveats
for colocalization analysis and how to use it.
JACoP is also a useful plugin, and it is described in this publication
https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2818.2006.01706.xI suspect you did not receive help because your question yesterday was
broad and vague, and it was not clear what you needed.
For more help in the future post specific questions or issues and check
out the forum
https://forum.image.sc/ . Searching for colocalization
there yields a lot of useful results.
https://forum.image.sc/search?q=colocalizationGood luck!
Nick
On 4/5/19 9:41 AM, Rohitesh Gupta wrote:
> Hi Guys,
>
> I had posted a query yesterday to which I did receive a reply. I am attaching an image which doesn't have any overlap between the green and red signal. It would be great if anyone can help in sharing the methodology to quantify an overlap (red and green making a yellow signal). In this case, the overlap factor should show zero value.
>
> Thanks,
> Rohitesh
>
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