Re: Quantifying overlap on fluorescence image

Posted by Krs5 on Apr 05, 2019; 4:41pm
URL: http://imagej.273.s1.nabble.com/Quantifying-overlap-on-fluorescence-image-tp5021990p5021993.html

Dear Rohitesh,

Nick gave already some good links to measure colocalization.

However, I don't agree with you that there is no overlap between the red and green signal in your image. Even by just looking at your jpg image (hope you have the original image for analysis as you lose information when using the jpg format) I can see already an overlap as some of the pixels show yellowish. Splitting the channels in red and green images show this as well, see attached.

Best wishes

Kees

Dr Ir K.R. Straatman
Senior Experimental Officer
Advanced Imaging Facility
Centre for Core Biotechnology Services
University of Leicester
www.le.ac.uk/advanced-imaging-facility



-----Original Message-----
From: ImageJ Interest Group <[hidden email]> On Behalf Of Rohitesh Gupta
Sent: 05 April 2019 16:42
To: [hidden email]
Subject: Quantifying overlap on fluorescence image

Hi Guys,

I had posted a query yesterday to which I did receive a reply. I am attaching an image which doesn't have any overlap between the green and red signal. It would be great if anyone can help in sharing the methodology to quantify an overlap (red and green making a yellow signal). In this case, the overlap factor should show zero value.

Thanks,
Rohitesh

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