Re: How to perform watershed on this image

Posted by Krs5 on Apr 09, 2019; 8:10am
URL: http://imagej.273.s1.nabble.com/How-to-perform-watershed-on-this-image-tp5022019p5022023.html

Dear Rohitesh,

The below macro code is doing more or less what you want on the image you submitted. No idea if it will work on other images you have but give it a go

title = getTitle();
run("Duplicate...", " ");
run("8-bit");
run("Bandpass Filter...", "filter_large=40 filter_small=3 suppress=None tolerance=5 autoscale saturate");
run("Find Edges");
run("Find Edges");
run("Find Edges");
run("Auto Local Threshold", "method=Contrast radius=15 parameter_1=0 parameter_2=0 white");
run("Select All");
run("Enlarge...", "enlarge=-1");
run("Crop");
run("Analyze Particles...", "size=25000-Infinity add");
selectWindow(title);
roiManager("Show All without labels");

Best wishes

Kees


Dr Ir K.R. Straatman
Senior Experimental Officer
Advanced Imaging Facility
Centre for Core Biotechnology Services
University of Leicester
www.le.ac.uk/advanced-imaging-facility

ImageJ workshops 15 and 16 July, Leicester, UK. For registration visit https://www2.le.ac.uk/colleges/medbiopsych/facilities-and-services/cbs/AIF/workshops/imagej-workshops-July-2019


-----Original Message-----
From: ImageJ Interest Group <[hidden email]> On Behalf Of Rohitesh Gupta
Sent: 09 April 2019 01:02
To: [hidden email]
Subject: Re: How to perform watershed on this image

Hi All,

This is the corrected image.

Thanks,
Rohitesh

--
ImageJ mailing list: https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Fimagej.nih.gov%2Fij%2Flist.html&amp;data=02%7C01%7Ckrs5%40leicester.ac.uk%7C35f0bbd965d044c506ce08d6bc7ed566%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C0%7C636903650269547352&amp;sdata=%2FpB3PijyzKOR1rdpAXgZC68LavHifZbyPHNoeBVeu0c%3D&amp;reserved=0

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html