Re: Define edges

Posted by retgar on Apr 09, 2019; 6:27pm
URL: http://imagej.273.s1.nabble.com/Define-edges-tp5021980p5022034.html

Hello, and first of all thank you for such promising result!
I have tried your method and results was really good, but still having a
little downsides sometimes.
Like for example, watershed sometimes crop one patricle on two halfs or
doesnt separate some particles at all
<http://imagej.1557.x6.nabble.com/file/t382279/11.jpg>
Also, i tried in a such way:
8-bit
subtract background
window/level - auto (or without this option whatsoever)
threshold
Fill holes
watershed

But one thing that i noticed, is that thresholding values sometimes may
vary, and i guess there is no way to do it automaticaly? And to answer your
question: i`m studying grow kinetics, so precision is the key, in order to
obtain sustainable results



--
Sent from: http://imagej.1557.x6.nabble.com/

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html