Re: Quantifying overlap on fluorescence image
Posted by
CARL Philippe (LBP) on
Apr 13, 2019; 5:37pm
URL: http://imagej.273.s1.nabble.com/Quantifying-overlap-on-fluorescence-image-tp5021990p5022076.html
Dear Rohitesh,
Did you give a try to the tool I indicated you on my mail from April 6th (see below)?
This tool will be working for whatever input picture you may have.
In order to use coloc2 you need to (only) have 8 bit pictures as inputs. Is this your case?
My best regards,
Philippe
Dear Kenneth, Rohitesh and Kees,
You could give a try to the following plugin:
http://punias.free.fr/ImageJ/Colocalization_Finder.jarwhich is an update of the following tool:
https://imagej.nih.gov/ij/plugins/colocalization-finder.htmlwhich new descriptions can be found here:
http://punias.free.fr/ImageJ/colocalization-finder.htmlHave nice week-end,
Philippe
Le Samedi 13 Avril 2019 18:23 CEST, Rohitesh Gupta <
[hidden email]> a écrit:
> You are thinking too deep into the fluorescent spectra of these channels. I am just trying to understand how to quantify individual samples if there is a difference between the intensity of green/red channels based on the difference in the antibody staining on that particular sample. To quantify overlap, I wanted to use coloc2. But, it seems like that because of the warning message, I am not sure how to depend on the output file ? So now i am thinking to go with the basics, and just calculate fluorescence intensity independently for each channel and report that. I am sure if two samples are compared having different fluorescence intensity, then that will show up. Certainly, I won't be able to quantify overlap.
>
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