Re: Quantifying overlap on fluorescence image

Posted by Herbie on Apr 14, 2019; 5:29pm
URL: http://imagej.273.s1.nabble.com/Quantifying-overlap-on-fluorescence-image-tp5021990p5022080.html

Good day,

please note that ImageJ-plugins and -macros differ in many aspects.

Plugins are always written in Java. Here is a good introduction:
<https://imagingbook.files.wordpress.com/2013/06/tutorial171.pdf>

The ImageJ-macro language is mainly meant for rapid prototyping, i.e. for
calling plugins in a desired order. Here is a description
<https://imagej.nih.gov/ij/developer/macro/macros.html>
and here is a commented list of built-in functions.
<https://imagej.nih.gov/ij/developer/macro/functions.html>

To start with writing plugins or macros you should consider using the
macro-recorder functionality of ImageJ. (Select either macro or java.)

HTH

Herbie


Rohitesh Gupta wrote

> Hi Guys,
>
> I had posted a query yesterday to which I did receive a reply. I am
> attaching an image which doesn't have any
> overlap between the green and red signal. It would be great if anyone can
> help in sharing the methodology to quantify an overlap (red and green
> making a yellow signal). In this case, the overlap factor should show zero
> value.
>
> Thanks,
> Rohitesh
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>
>
> BC Image.jpg (180K)
> &lt;http://imagej.1557.x6.nabble.com/attachment/5021990/0/BC%20Image.jpg&gt;

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