Re: Feature description of condensation
Posted by
Cardone, Giovanni on
Apr 26, 2019; 9:38am
URL: http://imagej.273.s1.nabble.com/Feature-description-of-condensation-tp5022129p5022130.html
Hi Thomas,
if you limit the analysis to just the protein content, a simple coefficient of variation, aka relative standard deviation, of the intensity should already be able to highlight the difference in a numerical manner.
I hope it helps.
Giovanni
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Lendl,Thomas
Sent: Freitag, 26. April 2019 11:17
To:
[hidden email]
Subject: Feature description of condensation
Dear all,
I need to quantify the amount of aggregation of a certain protein in nuclei. Visually the difference is obvious but how to put that into numbers? I thought about some kind of wavelet analysis since the fluctuations in the aggregated state have a different frequency but I didn't find the right tool or the right parameters. Has anybody encountered a similar problem?
https://www.dropbox.com/sh/jknltkw2jmyeg1x/AAApo_VMu2-fe3ssvxZJXZu3a?dl=0Best regards,
Thomas
………………………………..
Thomas Lendl
Image Analysis/FACS Specialist
IMP
Research Institute of Molecular Pathology Campus-Vienna-Biocenter 1
1030 Vienna, Austria
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