Posted by
Lendl,Thomas on
Apr 26, 2019; 10:25am
URL: http://imagej.273.s1.nabble.com/Feature-description-of-condensation-tp5022129p5022131.html
Hi Giovanni,
Thank you for the suggestion! I already tried that and there are differences in CV but not so pronounced. Both types show coarser intensity fluctuations (due to nucleoli and uneven intensity) that mess up the standard deviation.
-----Original Message-----
From: ImageJ Interest Group <
[hidden email]> On Behalf Of Cardone, Giovanni
Sent: Freitag, 26. April 2019 11:38
To:
[hidden email]
Subject: Re: Feature description of condensation
Hi Thomas,
if you limit the analysis to just the protein content, a simple coefficient of variation, aka relative standard deviation, of the intensity should already be able to highlight the difference in a numerical manner.
I hope it helps.
Giovanni
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Lendl,Thomas
Sent: Freitag, 26. April 2019 11:17
To:
[hidden email]
Subject: Feature description of condensation
Dear all,
I need to quantify the amount of aggregation of a certain protein in nuclei. Visually the difference is obvious but how to put that into numbers? I thought about some kind of wavelet analysis since the fluctuations in the aggregated state have a different frequency but I didn't find the right tool or the right parameters. Has anybody encountered a similar problem?
https://www.dropbox.com/sh/jknltkw2jmyeg1x/AAApo_VMu2-fe3ssvxZJXZu3a?dl=0Best regards,
Thomas
………………………………..
Thomas Lendl
Image Analysis/FACS Specialist
IMP
Research Institute of Molecular Pathology Campus-Vienna-Biocenter 1
1030 Vienna, Austria
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