Re: Feature description of condensation

Posted by Herbie on Apr 26, 2019; 11:18am
URL: http://imagej.273.s1.nabble.com/Feature-description-of-condensation-tp5022129p5022133.html

Good day Thomas,

you could try to enhance the differences between the states by
bandpass-filtering. You may start with the setting "large=10" and
"small=0" and everything else unchecked. With your images in 32bit I get

StdDev_a = 9.99;
StdDev_s = 8.01;

If this isn't sufficient I would try evaluating the Fourier Power
Spectra of both images and use the "Radial Profile"-plugin to get
descriptors.

Regards

Herbie

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Am 26.04.19 um 12:25 schrieb Lendl,Thomas:

> Hi Giovanni,
>
> Thank you for the suggestion! I already tried that and there are differences in CV but not so pronounced. Both types show coarser intensity fluctuations (due to nucleoli and uneven intensity) that mess up the standard deviation.
>
>
> -----Original Message-----
> From: ImageJ Interest Group <[hidden email]> On Behalf Of Cardone, Giovanni
> Sent: Freitag, 26. April 2019 11:38
> To: [hidden email]
> Subject: Re: Feature description of condensation
>
> Hi Thomas,
>
> if you limit the analysis to just the protein content, a simple coefficient of variation, aka relative standard deviation, of the intensity should already be able to highlight the difference in a numerical manner.
>
> I hope it helps.
> Giovanni
>
>
> -----Original Message-----
> From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Lendl,Thomas
> Sent: Freitag, 26. April 2019 11:17
> To: [hidden email]
> Subject: Feature description of condensation
>
> Dear all,
>
> I need to quantify the amount of aggregation of a certain protein in nuclei. Visually the difference is obvious but how to put that into numbers? I thought about some kind of wavelet analysis since the fluctuations in the aggregated state have a different frequency but I didn't find the right tool or the right parameters. Has anybody encountered a similar problem?
>
> https://www.dropbox.com/sh/jknltkw2jmyeg1x/AAApo_VMu2-fe3ssvxZJXZu3a?dl=0
>
> Best regards,
> Thomas
>
> ………………………………..
> Thomas Lendl
> Image Analysis/FACS Specialist
>  
> IMP
> Research Institute of Molecular Pathology Campus-Vienna-Biocenter 1
> 1030 Vienna, Austria
>  
>
>
>
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