Re: Intensity measurements by Gaussian peak fitting

Posted by WantobeImageAnalyst on May 05, 2019; 4:48pm
URL: http://imagej.273.s1.nabble.com/Intensity-measurements-by-Gaussian-peak-fitting-tp5022156p5022159.html

Thank you, Herbie.

I used 'Track mate' to detect my particles like the attached image based on
blob size and intensity threshold. I have coordinates of their centroid. And
I want to apply 2D and 3D Gaussian fitting of different shapes according to
each particle to discard noise.(Then, it will provide total or mean
intensity of the region of interest(?))

P.S.I cannot upload the whole image(3D, timelapse) because it exceeded the
file size limit. If you let me share the image through Google drive, I'll do
that for a better understanding.

<http://imagej.1557.x6.nabble.com/file/t382308/sample.jpg> of these
particles.



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