Re: Intensity measurements by Gaussian peak fitting

Posted by Herbie on May 05, 2019; 5:04pm
URL: http://imagej.273.s1.nabble.com/Intensity-measurements-by-Gaussian-peak-fitting-tp5022156p5022160.html

As I've written,

I doubt that you will get reliable results from such images.

Please post the result from trackmate and explain exactly how you've
set-up this plugin.

You don't reply at all to my suggestions and results. Why?

What about your claim that the images are diffraction-limited. How do
you test this?

What about the poor spatial resolution of your images?

What about previous knowledge of size and shape of your particles?

"WantobeImageAnalyst"
Are you serious?

Regards

Herbie

:::::::::::::::::::::::::::::::::::::::::::::::::
Am 05.05.19 um 18:48 schrieb WantobeImageAnalyst:

> Thank you, Herbie.
>
> I used 'Track mate' to detect my particles like the attached image based on
> blob size and intensity threshold. I have coordinates of their centroid. And
> I want to apply 2D and 3D Gaussian fitting of different shapes according to
> each particle to discard noise.(Then, it will provide total or mean
> intensity of the region of interest(?))
>
> P.S.I cannot upload the whole image(3D, timelapse) because it exceeded the
> file size limit. If you let me share the image through Google drive, I'll do
> that for a better understanding.
>
> <http://imagej.1557.x6.nabble.com/file/t382308/sample.jpg> of these
> particles.
>
>
>
> --
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>
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>

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