Re: Intensity measurements by Gaussian peak fitting

Posted by Arne Seitz on May 06, 2019; 10:50am
URL: http://imagej.273.s1.nabble.com/Intensity-measurements-by-Gaussian-peak-fitting-tp5022156p5022161.html

Dear Herbie,

I agree with the content of your replies but I think the tone is unappropriated.
Getting personally insulting shall to my understanding be avoided in such a forum.

Best regards
Arne


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Dr. Arne Seitz
Head of Bioimaging and Optics Platform (BIOP)
Swiss Institute of Technology (EPFL)
Faculty of Life Sciences
Station 15
CH-1015 Lausanne

Phone: [phone]
Fax:      [phone]
http://biop.epfl.ch/
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-----Original Message-----
From: ImageJ Interest Group <[hidden email]> On Behalf Of Herbie
Sent: Sonntag, 5. Mai 2019 19:04
To: [hidden email]
Subject: Re: Intensity measurements by Gaussian peak fitting

As I've written,

I doubt that you will get reliable results from such images.

Please post the result from trackmate and explain exactly how you've set-up this plugin.

You don't reply at all to my suggestions and results. Why?

What about your claim that the images are diffraction-limited. How do you test this?

What about the poor spatial resolution of your images?

What about previous knowledge of size and shape of your particles?

"WantobeImageAnalyst"
Are you serious?

Regards

Herbie

:::::::::::::::::::::::::::::::::::::::::::::::::
Am 05.05.19 um 18:48 schrieb WantobeImageAnalyst:

> Thank you, Herbie.
>
> I used 'Track mate' to detect my particles like the attached image
> based on blob size and intensity threshold. I have coordinates of
> their centroid. And I want to apply 2D and 3D Gaussian fitting of
> different shapes according to each particle to discard noise.(Then, it
> will provide total or mean intensity of the region of interest(?))
>
> P.S.I cannot upload the whole image(3D, timelapse) because it exceeded
> the file size limit. If you let me share the image through Google
> drive, I'll do that for a better understanding.
>
> <http://imagej.1557.x6.nabble.com/file/t382308/sample.jpg> of these
> particles.
>
>
>
> --
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>
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>

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