Re: measurement of fluorescence intensity over time

Posted by Herbie on Sep 25, 2019; 7:14pm
URL: http://imagej.273.s1.nabble.com/measurement-of-fluorescence-intensity-over-time-tp5022467p5022468.html

Good day María Victoria,

I guess you need the mean intensity in the circular selections for all
slices.

If so,

1.
make the desired selection on the first slice of your stack

2.
add the selection to the ROI Manager
(Analyze >> Tools >> ROI Manager >> Add)

3.
makes sure that in "Set Measurements" the "Mean gray value" is checked

4.
in the ROI Manager dialog go to "More >> Multi Measure"

5.
in the opening dialog check all options and click "OK"

6.
a Results table opens showing the desired mean values

HTH

Herbie

:::::::::::::::::::::::::::::::::::::::::::::::::
Am 25.09.19 um 20:27 schrieb María Victoria Pepe:

> Hi!
>
> I need to measure the fluorescence intensity within a circular selection
> and then, repeat the measurement in the same way over 800 slices (each one
> represents a time point)
>
> I would like to know how to create a macro that allows me to do this
> automatically
>
>
>
> Thanks for your help
>
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> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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