Re: measurement of fluorescence intensity over time

Posted by Michael Schmid on Sep 25, 2019; 8:21pm
URL: http://imagej.273.s1.nabble.com/measurement-of-fluorescence-intensity-over-time-tp5022467p5022469.html

Hi María Victoria,

if you have an image stack, you can simply use Image>Stacks>Plot z-Axis
Profile. No macro required.
Alternatively, use Image>Stacks>Measure Stack; it will give you the
values for whatever properties you have selected in Analyze>Set
Measurements. You will probably want "Mean".

Of course, you can record these commands (Plugins>Macros>Record) and use
them in a macro.


Michael
________________________________________________________________
On 25.09.19 20:27, María Victoria Pepe wrote:

> Hi!
>
> I need to measure the fluorescence intensity within a circular selection
> and then, repeat the measurement in the same way over 800 slices (each one
> represents a time point)
>
> I would like to know how to create a macro that allows me to do this
> automatically
>
>
>
> Thanks for your help
>
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> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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