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Re: measurement of fluorescence intensity over time

Posted by María Victoria Pepe on Sep 26, 2019; 2:15pm
URL: http://imagej.273.s1.nabble.com/measurement-of-fluorescence-intensity-over-time-tp5022467p5022472.html

Thank you very much for your answers
They were very helpful

El mié., 25 sept. 2019 a las 17:22, Michael Schmid (<[hidden email]>)
escribió:

> Hi María Victoria,
>
> if you have an image stack, you can simply use Image>Stacks>Plot z-Axis
> Profile. No macro required.
> Alternatively, use Image>Stacks>Measure Stack; it will give you the
> values for whatever properties you have selected in Analyze>Set
> Measurements. You will probably want "Mean".
>
> Of course, you can record these commands (Plugins>Macros>Record) and use
> them in a macro.
>
>
> Michael
> ________________________________________________________________
> On 25.09.19 20:27, María Victoria Pepe wrote:
> > Hi!
> >
> > I need to measure the fluorescence intensity within a circular selection
> > and then, repeat the measurement in the same way over 800 slices (each
> one
> > represents a time point)
> >
> > I would like to know how to create a macro that allows me to do this
> > automatically
> >
> >
> >
> > Thanks for your help
> >
> > --
> > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> >
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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