Posted by
CARL Philippe (LBP) on
Oct 04, 2019; 9:32am
URL: http://imagej.273.s1.nabble.com/no-subject-tp5022485p5022488.html
Dear Herbie,
What you are describing, i.e. of "geometrically flattening the fiber" this can very easily be done by the holographic microscope of Lyncee Tec:
https://www.lynceetec.com/Such a system is for example used in industry within quality control of the measurement of the roughness of beads used in pens.
Indeed if the roughness of such beads is too big, you will put too much ink on your paper and for a too smooth bead there will be not enough ink deposited on the paper.
So they take a 3D picture of the bead (with a nm resolution in z) flatten the surface by means of the bead curvature (similarly to the world maps) and then measure the surface roughness.
My best regards,
Philippe
Philippe CARL
Laboratoire de Bioimagerie et Pathologies
UMR 7021 CNRS - Université de Strasbourg
Faculté de Pharmacie
74 route du Rhin
67401 ILLKIRCH
Tel : +33(0)3 68 85 41 84
----- Mail original -----
De: "Herbie" <
[hidden email]>
À: "imagej" <
[hidden email]>
Envoyé: Vendredi 4 Octobre 2019 10:12:45
Objet: Re: Holes in a fiber
Dear poster,
obviously the holes are in a 3D-object (fiber) which means that the
holes suffer from geometric distortions in depth and their sizes can't
be accurately measured.
One way to overcome this problem is by geometrically flattening the
fiber but I doubt that this is possible with images of such low spatial
resolution.
Last but not least, the image appears to be copied from the literature.
Regards
Herbie
::::::::::::::::::::::::::::::::::::
Am 02.10.19 um 13:33 schrieb 闪闪小妖:
> [image: image.png]
> Dear
> I am very sorry to interrupt, how to calculate the size distribution of the
> holes in the figure, which plugin of image j to use? Thank you very much.
>
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