> Dear Herbie,
> What you are describing, i.e. of "geometrically flattening the fiber" this can very easily be done by the holographic microscope of Lyncee Tec:
>
https://www.lynceetec.com/> Such a system is for example used in industry within quality control of the measurement of the roughness of beads used in pens.
> Indeed if the roughness of such beads is too big, you will put too much ink on your paper and for a too smooth bead there will be not enough ink deposited on the paper.
> So they take a 3D picture of the bead (with a nm resolution in z) flatten the surface by means of the bead curvature (similarly to the world maps) and then measure the surface roughness.
>
> My best regards,
> Philippe
>
> Philippe CARL
> Laboratoire de Bioimagerie et Pathologies
> UMR 7021 CNRS - Université de Strasbourg
> Faculté de Pharmacie
> 74 route du Rhin
> 67401 ILLKIRCH
> Tel : +33(0)3 68 85 41 84
>
> ----- Mail original -----
> De: "Herbie" <
[hidden email]>
> À: "imagej" <
[hidden email]>
> Envoyé: Vendredi 4 Octobre 2019 10:12:45
> Objet: Re: Holes in a fiber
>
> Dear poster,
>
> obviously the holes are in a 3D-object (fiber) which means that the
> holes suffer from geometric distortions in depth and their sizes can't
> be accurately measured.
>
> One way to overcome this problem is by geometrically flattening the
> fiber but I doubt that this is possible with images of such low spatial
> resolution.
>
> Last but not least, the image appears to be copied from the literature.
>
> Regards
>
> Herbie
>
> ::::::::::::::::::::::::::::::::::::
> Am 02.10.19 um 13:33 schrieb 闪闪小妖:
>> [image: image.png]
>> Dear
>> I am very sorry to interrupt, how to calculate the size distribution of the
>> holes in the figure, which plugin of image j to use? Thank you very much.
>>
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