Re: Multi-stack registration of single-channel confocal images

Posted by wmacturk on Oct 09, 2019; 12:37am
URL: http://imagej.273.s1.nabble.com/Multi-stack-registration-of-single-channel-confocal-images-tp5022499p5022503.html

CARL Philippe (LBP) wrote
> Dear William,
> Please look at the following discussion:
> http://imagej.1557.x6.nabble.com/registering-a-stack-advice-needed-td5020546.html

Thanks for the reference Philippe. Unless I'm missing something, though, the
discussion doesn't seem to address my issue. I believe the main problem is
that, as these are confocal images, and the nucleolus is not a perfect
sphere (rather oblong, and imaged at an angle), the top slice shows a
portion that is noticeably up-and-right from the bottom slice, with no other
landmarks for registration. I'll attach two slices to show what I mean.

<http://imagej.1557.x6.nabble.com/file/t382446/DAPI_top.jpg>
<http://imagej.1557.x6.nabble.com/file/t382446/DAPI_bottom.jpg>



--
Sent from: http://imagej.1557.x6.nabble.com/

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html