Posted by Krs5 on Oct 09, 2019; 1:41pm URL: http://imagej.273.s1.nabble.com/Autophagy-Analysis-using-ImageJ-tp5022504p5022505.html
Dear Rohitesh,
This depends a little on your images and your cells. For example: Can you identify individual cells? Is there one vacuole/cell? Do you have a nuclear staining?
Maybe you can show a few images?
Best wishes
Kees
Dr Ir K.R. Straatman
Senior Experimental Officer
Advanced Imaging Facility
Centre for Core Biotechnology Services
University of Leicester
www.le.ac.uk/advanced-imaging-facility
-----Original Message-----
From: Rohitesh Gupta <[hidden email]>
Sent: 09 October 2019 14:26
Subject: Autophagy Analysis using ImageJ
Hi All,
I am writing after a long long time.
I have an image which has white dots inside the cells and I wanted to compare this image with another image which doesn't have white dots inside the cells. The objective is to count no. of cells with white dots (vacuoles) and not those cells where the vacuoles are absent. Please suggest which approach can be used on imagej.