Posted by
Herbie on
Oct 10, 2019; 4:20pm
URL: http://imagej.273.s1.nabble.com/Totally-new-here-Color-Counter-question-tp5022515p5022523.html
Good day Emily,
before you start thinking about any processing, you should be clear that you
can only consider leafs or parts thereof that actually are visible.
That said, I'm not sure what percentages you expect from images similar to
both of the provided samples in which the leafs overlap in an arbitrary
fashion.'m not sure if percentages measured from such samples are
meaningful.
Furthermore, you should avoid any additional structures that could be
recognized as leafs (according to their color) such as the ring-shaped metal
structure (which should better be white) and perhaps soil.
If you think that in spite of these concerns it is worth a try, then I
recommend to do a color transformation from RGB to CMYK.
You may get reasonable measures of the desired areas by thresholding the
color channels according to the automatic "RenyEntropy"-scheme. After
thresholding you can measure the selected area.
Yellow channel: Estimate of the whole leaf area
Magenta channel: Estimate of reddish leaf area
Cyan channel: Estimate of greenish leaf area
Please make sure that you understand what the above processing means but
first of all, re-think, whether such measurements make sense at all.
Regards
Herbie
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