Posted by
Charitra on
Oct 17, 2019; 9:03am
URL: http://imagej.273.s1.nabble.com/how-does-ImageJ-measure-circular-ROI-tp5022481p5022548.html
Hello Herbie and Michael
Thank you for your replies.
Herbie, I had posted my question on both the mailing list and the forum on
the same day. Not sure what you mean by reposting. I'm not very familiar
with how the forum and mailing list work, sorry.
But coming back, this was what I suspected - that ImageJ does not care about
sub-pixels. I am not interested in filling sub-pixel oval ROIs; I want to
measure them. So I just used 'fill' in a way to visualize which pixels were
going into the measurement. But coming back to Michael's answer, yes, the
values in the ROI managers are always integers, but if I 'specify' a ROI and
add it to the ROI manager, although the values are an integer, the sub-pixel
nature of the ROI is maintained. Or was this a wrong observation of mine?
Also, if there is no way to measure just 40% or 50% of the pixel, then I am
afraid I cannot use the oval ROIs. I have switched to using square ROIs
instead, as I cannot afford to do guesswork on these intensity measurements.
Moreover, in oval ROIs, the number of pixels taken into consideration is not
clear and I need to know exactly which and how many pixels are taken into
account. So I think I am going to continue with rectangular ROIs. Sorry for
the delay in replying, and have a nice day!
Charitra.
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