Posted by
CARL Philippe (LBP) on
URL: http://imagej.273.s1.nabble.com/Re-Colocalization-Finder-tp5022772p5022775.html
Dear Chin, Jeremy, Jérôme and all,
Please use the last version of the plugin under the following link:
http://punias.free.fr/ImageJ/colocalization-finder.htmland forget all the version I referenced previously, knowing that the features they implemented became obsolete by the last implemented developments.
For example, I got rid of the "Restrain selection to pixels..." feature of the "original version" knowing that you can now easily reproduce it by moving over the selection ROI within the scatterPlot.
And I had previously developped a Colocalization_Finder_with_ROI plugin so that it was possible for the user to set the starting selection ROI within the scatterPlot positions for a use within macro.
The new "on the fly" features illustrated by the two macro examples on the website are now way more powerful and versatile than this special version of the plugin.
Now coming back to your question, please correct me in the case I didn't correctly understand it.
But you were reporting that with the last version of the plugin it isn't anymore possible to have a rectangular selection ROI within the scatterPlot.
In fact looking carefully within your attached picture, (on it's left side scatterPlot) it is possible to see that there is actually a selection ROI within the scatterPlot, it is just covering all the scatterPlot graph.
I put this setting as default (following some biologists colleagues within my laboratory recommandations) and in the case the user deletes the ROI it is automatically regenerated at these positions.
Also you are not anymore restrained to a rectangular ROI within the scattedPlot (although I'm not sure whether such feature makes sense scientifically).
Nevertheless do to the automatic regeneration of the ROI I described higher, you need to go through either the roiManager or a macro command in order to create an oval ROI within the scatterPlot (there isn't such an issue for a Polygon or Freehand selection).
As the issue described by Jeremy, it seems serious and is more than probably a bug, really sorry about that!
However given that I'm quite sick at the moment with spending most of the time in bed, please allow me probably a couple days to seriously look into it and solve it.
Have a nice day,
Philippe
Philippe CARL
Laboratoire de Bioimagerie et Pathologies
UMR 7021 CNRS - Université de Strasbourg
Faculté de Pharmacie
74 route du Rhin
67401 ILLKIRCH
Tel : +33(0)3 68 85 41 84
----- Mail original -----
De: "chin" <
[hidden email]>
À: "imagej" <
[hidden email]>
Envoyé: Mercredi 11 Décembre 2019 08:21:40
Objet: Re: Colocalization Finder
Hi Philipppe,
There seems a problem on the latest version of "Colocalization_Finder.jar"
which cannot produce a
a rectangular selection on the diagram. While using the
"Colocalization_Finder_with_ROI.jar" you
mentioned on previous thread, it could provide spaces for parameter setting
and could produce the
adjustable rectangular selection on the diagram. Please have a look at the
figure.
<
http://imagej.1557.x6.nabble.com/file/t378371/%E6%9C%AA%E5%91%BD%E5%90%8D.jpg>
Thanks a lot.
chin
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