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Help to create macro

Posted by OBEID Patricia 154904 on Dec 12, 2019; 5:31am
URL: http://imagej.273.s1.nabble.com/Help-to-create-macro-tp5022778.html

Hello,
I want to write a macro allowing me to characterize inclusions in the cell nucleus (number / nucleus, size and intensity of fluorescence).
I think at first to segment the nuclei and then look inside this mask for the inclusions of interest.
My problem is that I do not know how to make a new segmentation in the segmentation of the nuclei.
Thank you in advance for your advice and ideas so that I can write this macro.
I attach an image of cores (DAPI) and an image with the marker of interest (TxRed).
A big thank you to all,
Patricia

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Patricia OBEÏD
Ingénieur de Recherche /Animateur Informatique Biomics
Institut de Recherche Interdisciplinaire de Grenoble
DRF / IRIG / DS /BGE U1038/ Biomics / Bât 4020
Commissariat à l'énergie atomique et aux énergies alternatives
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image001.jpg (11K) Download Attachment
s1_TxRed.tif (700K) Download Attachment
s1_DAPI.tif (700K) Download Attachment