Help to create macro
Posted by
OBEID Patricia 154904 on
Dec 12, 2019; 5:31am
URL: http://imagej.273.s1.nabble.com/Help-to-create-macro-tp5022778.html
Hello,
I want to write a macro allowing me to characterize inclusions in the cell nucleus (number / nucleus, size and intensity of fluorescence).
I think at first to segment the nuclei and then look inside this mask for the inclusions of interest.
My problem is that I do not know how to make a new segmentation in the segmentation of the nuclei.
Thank you in advance for your advice and ideas so that I can write this macro.
I attach an image of cores (DAPI) and an image with the marker of interest (TxRed).
A big thank you to all,
Patricia
_______________________________________
[
http://www.cea.fr/var/cea/signatures/cea_logo.jpg]<
http://www.cea.fr/>
Patricia OBEÏD
Ingénieur de Recherche /Animateur Informatique Biomics
Institut de Recherche Interdisciplinaire de Grenoble
DRF / IRIG / DS /BGE U1038/ Biomics / Bât 4020
Commissariat à l'énergie atomique et aux énergies alternatives
Email :
[hidden email]<mailto:
[hidden email]>
Tél : (+33)4 38 78 47 12 Fax : (+33)4 38 78 59 17
Toute notre actualité sur www.cea.fr et sur
http://www.cea.fr/drf/big/bge/biomics<
http://www.cea.fr>
<
http://www.cea.fr>Suivez-nous également sur Twitter : @CEA_Recherche<
https://twitter.com/#!/CEA_Recherche> et sur
https://twitter.com/BiomicsLab--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html