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Re: Help to create macro

Posted by OBEID Patricia 154904 on Dec 17, 2019; 3:28pm
URL: http://imagej.273.s1.nabble.com/Help-to-create-macro-tp5022778p5022791.html

Good evening Gabriel,
as proposed, here are the binarized and cleaned images (as best as I could).
Thank you very much for your help.
Cordially
Patricia

-----Message d'origine-----
De : ImageJ Interest Group <[hidden email]> De la part de Gabriel Landini
Envoyé : mardi 17 décembre 2019 15:38
À : [hidden email]
Objet : Re: Help to create macro

On Tuesday, 17 December 2019 13:44:41 GMT [hidden email] wrote:
> Hi Glyn,
> I'm actually interested in the intensity and size of each object
> contained in the nucleus. So I'm trying to adapt Gabriel's solution
> but I admit that I'm getting lost ... Thank you very much for your feedback.
> Regards,
> Patricia

If you get stuck with the RCC analysis, please binarise (and clean) the images of the nuclei and the bright dots in the nuclei and post them here. I can then attempt to show you how I think this can be resolved using macros calling the RCC plugin(s).

Cheers

Gabriel

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s1_TxRed_binary.tif (350K) Download Attachment
s1_DAPI_binary.tif (350K) Download Attachment