HELP!!!-segmentation and quantification of DIC images

Posted by Hui Zhang on
URL: http://imagej.273.s1.nabble.com/HELP-segmentation-and-quantification-of-DIC-images-tp5022977.html

<http://imagej.1557.x6.nabble.com/file/t382540/DIC-droplets.png> Hi

I am a phD student in Germany. recently I am focusing on the phase
separation property of a protein.
As tested the GFP tag can enhance the ability of the protein to phase
sepatate, thus forming bigger droplets.
So I can only purified and utilize the untaged protein for assays and
generate a series of two dimentional DIC images with round shapes.
now the problem is that I need to quantify the number, size and roundess
(aspect ratio), which requires a good program to segmentate.
I attached an image also in case you want to have a look. you can see that
the background is not even, maybe it is not qualified, Do I need to generate
new images?

does someone of you know how to process or have some good program, software,
or plug-in that I can apply.

Best wishes
Hui Zhang



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