Posted by
Michael Schmid on
Feb 27, 2020; 5:05pm
URL: http://imagej.273.s1.nabble.com/HELP-segmentation-and-quantification-of-DIC-images-tp5022977p5022979.html
Hi Hui,
I guess that it can be done with a bit of programming:
(1) High pass filter to get the background flat
https://imagejdocu.tudor.lu/plugin/filter/highpass_filter/start(2) Programming:
For each diagonal line (in upward direction), replace the pixel value
with the running sum over the pixels from the beginning to the current
point. Then subtract the average of that line.
Alternatives without diagonal lines:
Either have illumination from the left and do the same with the "usual"
horizontal image lines,
or do the operation for columns and lines, and sum/subtract these two
results.
Michael
________________________________________________________________
On 27.02.20 12:52, Hui Zhang wrote:
> <
http://imagej.1557.x6.nabble.com/file/t382540/DIC-droplets.png> Hi
>
> I am a phD student in Germany. recently I am focusing on the phase
> separation property of a protein.
> As tested the GFP tag can enhance the ability of the protein to phase
> sepatate, thus forming bigger droplets.
> So I can only purified and utilize the untaged protein for assays and
> generate a series of two dimentional DIC images with round shapes.
> now the problem is that I need to quantify the number, size and roundess
> (aspect ratio), which requires a good program to segmentate.
> I attached an image also in case you want to have a look. you can see that
> the background is not even, maybe it is not qualified, Do I need to generate
> new images?
>
> does someone of you know how to process or have some good program, software,
> or plug-in that I can apply.
>
> Best wishes
> Hui Zhang
--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html