Re: HELP!!!-segmentation and quantification of DIC images

Posted by Kenneth Sloan-2 on
URL: http://imagej.273.s1.nabble.com/HELP-segmentation-and-quantification-of-DIC-images-tp5022977p5022989.html

When I last had to deal with DIC images (mid 1980s), my most successful approach was to separately identify the light and dark halves (sometimes using, and sometimes deriving, the directionality) and then merging the two sets of objects into matched pairs.

Alas, this was long before FIJI existed, so I can't offer a plugin (I'm not sure I still have the code).  

Note that this required considerable processing one level above pure "image processing".

An alternative idea might be template matching (at a range of scales matching the expected range of sizes of your particles).

Finally...the boundaries separating the light halves from the dark halves are often the strongest edges in the image.  If you combine that with information on the directionality (or, perhaps estimate it), it might suffice to find and characterize (by length) these boundaries.  This may depend on how variable the objects are in their shape.  If you are able to assume they are all circular, then the separating boundary completely characterizes each object.

I'm very interested in Michael's new plugin - is it possible to get a quick gloss on the methods used?  My interest these days (actually, a potential future interest) is in "split-detector" adaptive optics images (which look like DIC to me, but the people who do this sort of thing don't seem to call it that).

--
Kenneth Sloan
[hidden email]
Vision is the art of seeing what is invisible to others.

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