Re: HELP!!!-segmentation and quantification of DIC images

Posted by Till Bretschneider-2 on
URL: http://imagej.273.s1.nabble.com/HELP-segmentation-and-quantification-of-DIC-images-tp5022977p5022993.html

You can try our QuimP plugin for ImageJ which has a DIC preprocessing plugin. I tried it on your example and it works reasonably well (Decay factor 0.2, all other parameters unchanged).

Using QuimP you can also measure the size of your droplets.
The main question is why not use the fluorescence channel if your droplets are GFP tagged.
Also, it looks like the quality of your DIC image can be improved.
Best wishes,
Till

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html