Re: HELP!!!-segmentation and quantification of DIC images

Posted by Hui Zhang on
URL: http://imagej.273.s1.nabble.com/HELP-segmentation-and-quantification-of-DIC-images-tp5022977p5023012.html

Hi Till,

As I tested in my case, the GFP tag could effect the size of droplets
compared with untagged protein. So I plan latter to do all such experiments
using the wild type and mutated proteins with no tags (maybe there are some
other tags for choice, but really time comsumming to test).
as for the quality of DIC images, I have no clear idea which kind is the
best, what I could image is merely clear droplets with even background,
which I am trying to improve recently.

Best,
Hui



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