Posted by Krs5 on URL: http://imagej.273.s1.nabble.com/Specifying-a-predetermined-ROI-tp5023001p5023018.html
Dear Abdul,
If you have already a selection you can skip the first 3 lines in macro 1 and the first 4 lines in macro 2 and start with roiManager("add"); It is doing the same as in Herbie's macro code but does not require the turn the image. Check if the number of pixels is equal to the 200 µm required.
Best wishes
Kees
-----Original Message-----
From: ImageJ Interest Group <[hidden email]> On Behalf Of Abdulhameed Bakreen
Sent: 05 March 2020 14:41
To: [hidden email] Subject: Re: Specifying a predetermined ROI
Dear Kees,
Thank you for your macros. However, I have not been able to bring them to work as they're requesting I select the ischemic border after running them, even when it's already selected. Have I missed some steps?
Anyways, the macro from Herbie has been working very well for me, and I will proceed with it.
Once again, I appreciate your effort, Kees. Thanks.